The research is focused on the spectroscopic characterization of mediator radicals formed during the catalytic mechanism of laccase.
Laccase-mediator systems are used in a wide range of applications for biotechnological purposes. However the use of such molecules is limited by their costs, toxicity of by-products and high mediator/laccase molar ratio. Furthermore most of mediators seem to be "false mediators" as they are fully oxidised and depleted from the cycle. The study proposes to find good mediator molecules through a spectroscopic characterization of the enzymatic mechanism and the radical intermediates formed during the process.